try p a-b d l , yA2, AcrAI, metGI, lacA1, choA1 (we.e., lack to possess p-aminobenzoic acidic, choline, methionine; lactose nonutilizing; acnflavin unwilling; purple conidiospores).
A sterile mug Petri pan (grease-free). a dozen tubes which have 0.8 mL saline towards frost (to your examples). 24 dishes CMT (= CM that have 0.01%Triton X-one hundred to reduce colony proportions). a dozen plates SM-methionine (= MM+cho+pab, to get to know the new deficiencies of WG282). dos pm) to your Coulter prevent.
Prepare 14 mL spore suspension when you look at the saline-Tween for the a thirty-mL shag-cap bottles. Assemble new eris nasÄ±l kullanÄ±lÄ±yor spores regarding the plate and avoid discharge of spores throughout the room. Move intensely to split the fresh new conidiospore organizations. Filter by way of a little cotton fleece plug in a funnel so you’re able to lose mycelium particles. 繼續閱讀 Particle-free saline (filtered by way of a membrane layer filter out 0