try p a-b d l , yA2, AcrAI, metGI, lacA1, choA1 (we.e., lack to possess p-aminobenzoic acidic, choline, methionine; lactose nonutilizing; acnflavin unwilling; purple conidiospores).
A sterile mug Petri pan (grease-free). a dozen tubes which have 0.8 mL saline towards frost (to your examples). 24 dishes CMT (= CM that have 0.01%Triton X-one hundred to reduce colony proportions). a dozen plates SM-methionine (= MM+cho+pab, to get to know the new deficiencies of WG282). dos pm) to your Coulter prevent.
Prepare 14 mL spore suspension when you look at the saline-Tween for the a thirty-mL shag-cap bottles. Assemble new eris nasıl kullanılıyor spores regarding the plate and avoid discharge of spores throughout the room. Move intensely to split the fresh new conidiospore organizations. Filter by way of a little cotton fleece plug in a funnel so you’re able to lose mycelium particles. 繼續閱讀 Particle-free saline (filtered by way of a membrane layer filter out 0